BG11培養基(+N/-N)

參考文獻:Stanier,?R.Y.,?Kunisawa,?R.,?Mandel,?M.&?Cohen-Bazire,?G.1971.?Purification?and?properties?of?unicellular?blue-green?algae?(Order?Chroococcales).?Bacteriol.?Rev.?35:?171-205.

上海光語生物科技有限公司提供配置好的bg11培養液,如需節省時間請聯系我們。

準備儲備液Stocks見下表:

Stock1 試劑 per?1000mL? per?500mL
NaNO3(+N)/NaCl(-N) 30.0g 15.0g
1 K2HPO4?/?K2HPO4·3H2O 0.8g?/?1.05g 0.4g?/?0.53g
2 MgSO4·7H2O 1.5g 0.75g

 

Stock2 試劑 per?100mL? per?200mL
3 檸檬酸/一水合檸檬酸C6H8O7 0.30g/0.33g 0.60/0.66g
3 檸檬酸鐵銨:FeC6H5O7.NH4OH 0.30g 0.60g
3 Na2EDTA/Na2EDTA·2H2O 0.05g/0.055g 0.10g/0.11g

 

Stock3 試劑 per?100mL? per?200mL
4 CaCl2·2H2O/?CaCl2 1.80g/1.36g 3.6g/2.72g

 

Stock4 試劑 per?100mL? per?200mL
5 Na2CO3 2.00g 4.00g

 

Stock5 微量 per?1000mL? per?500mL
H3BO3 2.86g 1.43g
MnCl2·4H2O 1.81g 0.91g
ZnSO4·7H2O 0.22g 0.11g
Na2MoO4·2H2O 0.39g 0.20g
CuSO4·5H2O 0.08g 0.04g
Co(NO3)2?·6H2O/CoCl2?·6H2O 0.05g/0.04g 0.025g/0.02g

培養基配置:

Medium ? per?1000mL
Stock?solution?1 50.0mL
Stock?solution?2 2.0mL
Stock?solution?3 2.0mL
Stock?solution?4 1.0mL
Stock?solution?5 1.0mL

NaOHHCl調節pH值到7.1,固體培養基加1.5%的瓊脂粉。121℃,0.1Mpa,滅菌20min。

適用藻種:

BG11+N):浮游顫藻、萊茵衣藻、小球藻、集胞藻、銅綠微囊藻469、銅綠微囊藻915、水華束絲藻、水生所、密云;

BG11-N):魚腥藻7120、發菜藻、水華束絲藻

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